AN “IN VITRO” AND “IN VIVO” STUDY ON THE SAFETY OF LANOLIDE
Author Maria Grazia Angelini
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Tags Lanolide, lang_en, cytotoxic activity, primary irritant activity, rat keratinocytes, skin wound healing, V79 cells, lanolin, acute systemic toxicity, cytostatic activity
Lanolide, a synthetic substitute for lanolin has been tested for tolerability in a battery of in vitro and in vivo assays. The evaluation of its cytotoxic activity, carried out in V79 cells, an established line of Chinese hamster lung fibroblasts, using the neutral red, the trypan blue, and the MTT tetrazolium assays, gave concordant results which indicate that Lanolide can be considered practically atoxic at the highest soluble concentration. A dose-dependent cytostatic effect was observed after continuous exposure of the same cells, and a possible reduction of protein synthesis was suggested by a preliminary assay on rat keratinocytes, but in both cases active doses were higher that those present at the level of epidermal cells during normal usage conditions. In good agreement with these in vitro findings any meaningful toxic effect was absent in mice given 10 g/kg of Lanolide by the intraperitoneal route, no ocular lesions were present in rabbits after topical application of 200 mg, and neither primary irritant activity nor skin wound healing inhibition were observed in the same species.